Tuesday, June 3, 2014

Day 1 Questions



-        What did you do today?   
 o   Today, I learnt how to cultivate and isolate bacteria. I also learnt about lab safety rules and how to handle spirit lamps. I first used 70% ethanol to sterilise our gloves and workbench. Taking a marker, I marked half of the agar plates to be a control and the other half to be the part which is going to be experimented on. Then, taking a cotton swab, I dipped it into the mixture of bacteria culture and dabbed it onto the agar plates. Before and after using the cotton swabs and the inoculating loops, I had to use the methylated spirit lamps to kill of the bacteria found on it. After that, we had to wait for the next day to check on the growth of the bacteria.
-        What did you learn today?  
  o   I learnt how and what to be take precaution of. I also learnt on wearing proper lab coats and gloves. I learnt that bacteria is found everywhere and unexpected places could have even more bacteria than a toilet bowl.
-        How do you feel about today’s activities?
    o   This project has taught me how to handle equipment with care and to follow instructions carefully, for a simple mistake could affect the experimental results greatly.
    o   Bacteria can be grown to benefit people, and not all bacteria is harmful. Learning how to grow bacteria and not contaminating the area would reduce the risks of misusing bacteria.


What are bacteria? List down some characteristics of bacteria.

Bacteria are unicellular microorganisms found everywhere. Some characteristics of bacteria are that they are unicellular, can only be viewed under the micro scope and some have cell walls while some do not.

How can bacteria be identified?
Bacteria can be identified by staining(gram). The bacteria are stained with violet dye, then stained again with Potassium Iodide. Afterwards, they are washed with alcohol. The bacteria which lose this stain are gram negative while does who retain the stains are gram positive.

Name three common bacteria and places where they are commonly found.
Lactobacillus delbrueckii subsp. bulgaricus(yoghurt)
Lactobacillus acidophilus(vitagen)
 C. difficile(toilet water)

Are all bacteria harmful? If not, name some bacteria beneficial to humans.
No. Lactobacillus delbrueckii subsp. bulgaricus turns milk into yoghurt. Yoghurt smooths skin and builds calcium.

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