Thursday, June 5, 2014

3rd Day Questions

-        Plenary Sessions
Not all bacteria are harmful.
Oil can be used on microscopes at 100x to zoom in even more.
Lab equipments should not be brought out of the lad to reduce contamination.

-        How do you feel about the SST-NTU Flagship programme?
The SST-NTU Flagship programme is very meaningful and allows me to understand more about the facilities of NTU. This programme allowed me to experience something unique that will not normally be learned at Secondary level in the normal learning experiences.

This programme is very interesting and allowed me to broaden my knowledge. The programme not only allows me to understand my own programme, but even a brief overview of all the other programmes that were available.

Wednesday, June 4, 2014

Day 2 Questions

What did you do today?    o   Briefly describe your day’s activities-        I learned about how to setup microscopes and how to fine tune it. I also found out the results to our experiments and learned more about gram staining. To finish it off, my group presented to the class of our findings and learning experiences.
    o   Discuss any learning points from today’s activities
I learnt on the benefits of bacteria and how deadly it can be. I also learnt how to stain the bacteria to classify them into gram positive and gram negative. If the bacteria is positive, it can be more easily killed by antibiotics, while gram negative bacteria is harder to be killed.-        How do you feel about today’s activities?    o  This project taught me about not to abuse antibiotics and the consequences if we abuse them(unfinished course). If we do not complete the course, the bacteria might mutate and build up resistance to the antibiotics that were normally used to treat them. If not for today's activities, I would not have realised that bacteria could build up resistance to a point where even high concentrations of the antibiotics would not kill them.

Tuesday, June 3, 2014

Day 1 Questions



-        What did you do today?   
 o   Today, I learnt how to cultivate and isolate bacteria. I also learnt about lab safety rules and how to handle spirit lamps. I first used 70% ethanol to sterilise our gloves and workbench. Taking a marker, I marked half of the agar plates to be a control and the other half to be the part which is going to be experimented on. Then, taking a cotton swab, I dipped it into the mixture of bacteria culture and dabbed it onto the agar plates. Before and after using the cotton swabs and the inoculating loops, I had to use the methylated spirit lamps to kill of the bacteria found on it. After that, we had to wait for the next day to check on the growth of the bacteria.
-        What did you learn today?  
  o   I learnt how and what to be take precaution of. I also learnt on wearing proper lab coats and gloves. I learnt that bacteria is found everywhere and unexpected places could have even more bacteria than a toilet bowl.
-        How do you feel about today’s activities?
    o   This project has taught me how to handle equipment with care and to follow instructions carefully, for a simple mistake could affect the experimental results greatly.
    o   Bacteria can be grown to benefit people, and not all bacteria is harmful. Learning how to grow bacteria and not contaminating the area would reduce the risks of misusing bacteria.


What are bacteria? List down some characteristics of bacteria.

Bacteria are unicellular microorganisms found everywhere. Some characteristics of bacteria are that they are unicellular, can only be viewed under the micro scope and some have cell walls while some do not.

How can bacteria be identified?
Bacteria can be identified by staining(gram). The bacteria are stained with violet dye, then stained again with Potassium Iodide. Afterwards, they are washed with alcohol. The bacteria which lose this stain are gram negative while does who retain the stains are gram positive.

Name three common bacteria and places where they are commonly found.
Lactobacillus delbrueckii subsp. bulgaricus(yoghurt)
Lactobacillus acidophilus(vitagen)
 C. difficile(toilet water)

Are all bacteria harmful? If not, name some bacteria beneficial to humans.
No. Lactobacillus delbrueckii subsp. bulgaricus turns milk into yoghurt. Yoghurt smooths skin and builds calcium.

Sunday, May 18, 2014

Pre-camp

-What sounds interesting about this project?
Understanding and finding out what we are putting in our mouths(utensils) is interesting about this project.

-What do your think you can learn from this project?
I think I can learn ways to counter the bacteria or find out more about the bacteria and try to prevent the bacteria from infecting the user.

-Any immediate questions regarding the project?
What are the different kinds of bacteria that can be found? What is the safest measures to kill the bacteria?